An Integrated Systems Biology Approach to Understanding the Rules of Keratinocyte Colony Formation

by Tao Sun, Phil McMinn, Simon Coakley, Mike Holcombe, Rod Smallwood, and Sheila MacNeil

Journal of the Royal Society Interface, 2007



Closely coupled in vitro and in virtuo models have been used to explore the self-organization of normal human keratinocytes (NHK). Although it can be observed experimentally, we lack the tools to explore many biological rules that govern NHK self-organization. An agent-based computational model was developed, based on rules derived from literature, which predicts the dynamic multicellular morphogenesis of NHK and of a keratinocyte cell line (HaCat cells) under varying extracellular Ca++ concentrations. The model enables in virtuo exploration of the relative importance of biological rules and was used to test hypotheses in virtuo which were subsequently examined in vitro. Results ... [more]


Reference

Tao Sun, Phil McMinn, Simon Coakley, Mike Holcombe, Rod Smallwood, and Sheila MacNeil. An Integrated Systems Biology Approach to Understanding the Rules of Keratinocyte Colony Formation. Journal of the Royal Society Interface, vol. 4, no. 17, pp. 1077–1092, 2007


Bibtex Entry
@article{Sun2007,
  author  = "Sun, Tao and McMinn, Phil and Coakley, Simon and Holcombe, Mike and Smallwood, Rod and MacNeil, Sheila",
  title   = "An Integrated Systems Biology Approach to Understanding the Rules of Keratinocyte Colony Formation",
  journal = "Journal of the Royal Society Interface",
  volume  = "4",
  number  = "17",
  pages   = "1077--1092",
  year    = "2007"
}