Agent Based Modelling Helps in Understanding the Rules by Which Fibroblasts Support Keratinocyte Colony Formation

by Tao Sun, Phil McMinn, Mike Holcombe, Rod Smallwood and Sheila MacNeil

PLoS ONE, 2008



Background: Autologous keratincoytes are routinely expanded using irradiated mouse fibroblasts and bovine serum for clinical use. With growing concerns about the safety of these xenobiotic materials, it is desirable to culture keratinocytes in media without animal derived products. An improved understanding of epithelial/mesenchymal interactions could assist in this. Methodology/Principal Findings: A keratincyte/fibroblast o-culture model was developed by extending an agent-based keratinocyte colony formation model to include the response of keratinocytes to both fibroblasts and serum. The model was validated by comparison of the in virtuo and in vitro multicellular behaviour of keratinocytes and fibroblasts in single and co-culture in Greens ... [more]


Reference

Tao Sun, Phil McMinn, Mike Holcombe, Rod Smallwood and Sheila MacNeil. Agent Based Modelling Helps in Understanding the Rules by Which Fibroblasts Support Keratinocyte Colony Formation. PLoS ONE, vol. 3, no. 5, 2008


Bibtex Entry
@article{Sun2008,
  author  = "Sun, Tao and McMinn, Phil and Holcombe, Mike and Smallwood, Rod and MacNeil, Sheila",
  title   = "Agent Based Modelling Helps in Understanding the Rules by Which Fibroblasts Support Keratinocyte Colony Formation",
  journal = "PLoS ONE",
  volume  = "3",
  number  = "5",
  year    = "2008"
}